A Clinical Method for the Analysis of Amygdalin in Human Plasma
- 1 January 1978
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 11 (9) , 753-764
- https://doi.org/10.1080/00032717808059725
Abstract
A rapid, simple and sensitive method for the analysis of amygdalin in human plasma has been developed. The method is based on the hydrolytic action of the enzyme 6-glucosidase on amygdalin to yield a molar equivalent amount of benzaldehyde (along with 2 moles of glucose and a mole of HCN). The benzaldehyde is extracted from plasma with chloroform and determined by GLC using a flame ionization detector. The benzaldehyde recovered, when the method was applied to human plasma spiked with amygdalin, was found to be 9.5% of the added amygdalin. Such recovery was highly reproducible (± 1.5%) for amygdalin concentrations ranging from 2 to 20 μg/ml.Keywords
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