Detection and Typing of Borrelia burgdorferi Sensu Lato in Ixodes ricinus Ticks Attached to Human Skin by PCR

Abstract
Live Ixodes ricinus ticks attached to humans residing in Germany were examined for borreliae by dark-field microscopy and PCR. Borrelia species were identified by 16S rRNA sequence analysis, which showed the presence of several species, some not yet defined, and a high prevalence of multiply infected ticks.

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