Electrochemical Detector for Liquid Chromatography

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Abstract
An electrochemical detector for liquid chromatography has been developed for quantitatively detecting and identifying electroreducible components in eluates. The detector has a mercury-plated platinum wire cathode and was evaluated mainly with a reverse-phase system employing a 50:50 methanol-water carrier solvent containing 0.05M LiClO4 electrolyte. The limit of detection of the detector is shown to be at least as great as that for an ultraviolet photometric detector. Detector selectivity is demonstrated in experiments where the applied potential is chosen to discriminate between eluting components with different reduction potentials. The reduction potential of an eluting component and other qualitative information can be obtained from voltammetric scans performed with the trapped eluting component in the detector using a stopped-flow technique. A porous-plug electrode detector which provides greater electrolysis of an eluting component is also discussed in addition to general detector design considerations.

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