Yeast Srp1p has homology to armadillo/plakoglobin/beta-catenin and participates in apparently multiple nuclear functions including the maintenance of the nucleolar structure.
- 19 July 1994
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (15) , 6880-6884
- https://doi.org/10.1073/pnas.91.15.6880
Abstract
SRP1, a suppressor of certain temperature-sensitive mutations in RNA polymerase I in Saccharomyces cerevisiae, encodes a protein that is associated with nuclear pores. By using a system of conditional SRP1 expression and by isolating temperature-sensitive srp1 mutants, we have demonstrated that Srp1p is essential for maintenance of the crescent-shaped nucleolar structure, RNA transcription, and the proper functions of microtubules as inferred from analysis of nuclear division/segregation and immunofluorescence microscopy of microtubules. Different mutant alleles showed significantly different phenotypes in relation to these apparently multiple functional roles of the protein. We have also found that eight imperfect 42-amino-acid tandem repeats present in Srp1p are similar to the 42-amino-acid repeats in armadillo/plakoglobin/beta-catenin proteins present in adhesive junction complexes of higher eukaryotes. We discuss this similarity in connection with the observed pleiotropic effects of srp1 mutations.Keywords
This publication has 19 references indexed in Scilit:
- A repeating amino acid motif shared by proteins with diverse cellular rolesPublished by Elsevier ,1994
- Suppressor analysis of temperature-sensitive RNA polymerase I mutations in Saccharomyces cerevisiae: suppression of mutations in a zinc-binding motif by transposed mutant genes.Molecular and Cellular Biology, 1991
- Suppressor analysis of temperature-sensitive mutations of the largest subunit of RNA polymerase I in Saccharomyces cerevisiae: a suppressor gene encodes the second-largest subunit of RNA polymerase I.Molecular and Cellular Biology, 1991
- BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin.The Journal of cell biology, 1990
- The segment polarity gene armadillo encodes a functionally modular protein that is the Drosophila homolog of human plakoglobinCell, 1990
- Molecular cloning and amino acid sequence of human plakoglobin, the common junctional plaque protein.Proceedings of the National Academy of Sciences, 1989
- EXPRESSION OF A HUMAN P-450IIC GENE IN YEAST-CELLS USING GALACTOSE-INDUCIBLE EXPRESSION SYSTEM1989
- Molecular analysis of the armadillo locus: uniformly distributed transcripts and a protein with novel internal repeats are associated with a Drosophila segment polarity gene.Genes & Development, 1989
- Isolation and characterization of temperature-sensitive mutations in RPA190, the gene encoding the largest subunit of RNA polymerase I from Saccharomyces cerevisiae.Molecular and Cellular Biology, 1988
- A comprehensive set of sequence analysis programs for the VAXNucleic Acids Research, 1984