Protein Fusion: A Novel Reaction in Bacteriophage λ Head Assembly
- 1 April 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (4) , 1451-1455
- https://doi.org/10.1073/pnas.71.4.1451
Abstract
Parts of two phage-coded head proteins, pE and pC, become fused during bacteriophage lambda head assembly. pE is the main structural component of lambda heads and pC is a minor head protein that is not found as such in mature heads. The bond joining the two proteins appears to be covalent and is not a disulfide bond. Only a specific subset of the sequences of each protein is found in the fusion products, and these sequences are found in the products in equimolar amounts. Two nearly identical fusion products; X1 and X2, are detected; X2 is slightly smaller than X1 and appears to be a proteolytic cleavage product of X1. The fusion reaction probably takes place on a nascent head structure.Keywords
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