In Vitro Assay of Erythropoietin in Fetal Mouse Liver Cultures.

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Abstract
Fe bound to human transferrin, but not apotransferrin, increases the erythropoietin effect in stimulating incorporation of 3H-thymidine [3H-T] into DNA in fetal mouse liver cells in vitro. The erythropoietin effect, with or without transferrin-Fe, is blocked by erythropoietin pre-incubation with rabbit antierythropoietin serum. Human sera contain factors in addition to erythropoietin and transferrin-Fe which may modify the stimulation of incorporation of 3H-T into fetal mouse liver DNA induced by erythropoietin. Heat treatment of sera at 56.degree. C for 30 min does not necessarily destroy these factors. Acid heat treatment of sera (pH 5.5 and 100.degree. C for 5 min) may destroy inhibitory factors and can result in an apparent increase in serum erythropoietin activity assessed in this fetal mouse liver system.