Disc Electrophoretic Separation of Elongated Plant Viruses in Polyacrylamide-agarose Gels

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Abstract
Disc electrophoresis in polyacrylamide gels has often been used for investigation of virus proteins (McCarthy, 1968; Sarkar & Schilde-Rentschler, 1968; Semancik, 1966), but has seldom been used to separate whole viruses (Semancik, 1966; Tiselius, Hjerten & Jerstedt, 1965). Tiselius et al. (1965) were the first to fractionate a plant virus, turnip yellow mosaic virus (TYMV) by electrophoresis in polyacrylamide gels. Semancik (1966) and Niblett & Semancik (1969, 1970) used this method for fractionation and characterization of the components of purified isometric plant viruses, but separation in polyacrylamide gel was not possible for viruses with elongated particles, such as tobacco mosaic virus (TMV) or tobacco rattle virus. In this paper we report the electrophoresis of viruses with elongated particles in polyacrylamide-agarose gels of low polyacrylamide concentration. This method has been used before to fractionate nucleic acids and ribosomes (Peacock & Dingman, 1968; Dahlberg, Dingman & Peacock, 1969).

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