Immunoglobulin G (IgG) and IgA, but also Nonantibody Factors, Account for In Vitro Neutralization of Human Immunodeficiency Virus (HIV) Type 1 Primary Isolates by Serum and Plasma of HIV-Infected Patients
Open Access
- 1 June 2001
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 75 (11) , 5421-5424
- https://doi.org/10.1128/jvi.75.11.5421-5424.2001
Abstract
The factors present in serum and plasma samples of human immunodeficiency virus (HIV)-infected patients that are responsible for the neutralization of four HIV type 1 (HIV-1) primary isolates in vitro have been analyzed. Purification of immunoglobulins (Ig) by affinity chromatography showed that the activities were mostly attributable to IgG and less frequently to IgA. For two samples, we have shown that the high-level and broad-spectrum inhibitory activity was essentially caused by non-Ig factors interfering with the measurement of antibody-specific neutralizing activity.Keywords
This publication has 26 references indexed in Scilit:
- Antibody-Mediated Neutralization of Primary Human Immunodeficiency Virus Type 1 Isolates: Investigation of the Mechanism of InhibitionJournal of Virology, 2001
- Identification and characterization of sera from HIV-infected individuals with broad cross-neutralizing activity against group M (env clade A-H) and group O primary HIV-1 isolates.2000
- Mucosal and plasma IgA from HIV-exposed seronegative individuals neutralize a primary HIV-1 isolateAIDS, 2000
- Neutralization of Human Immunodeficiency Virus Type 1 (HIV‐1) Mediated by Parotid IgA of HIV‐1–Infected PatientsThe Journal of Infectious Diseases, 2000
- CD8+Cell‐Derived Anti–Human Immunodeficiency Virus Inhibitory FactorThe Journal of Infectious Diseases, 1999
- Neutralization of Primary Human Immunodeficiency Virus Type 1 Isolates: A Study of Parameters Implicated in Neutralizationin VitroAIDS Research and Human Retroviruses, 1997
- The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entryNature, 1996
- Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8 + T CellsScience, 1995
- Preparation and Characterization of Human HIV Type 1 Neutralizing Reference SeraAIDS Research and Human Retroviruses, 1995
- Serum IgA Subclasses and Molecular Forms in HIV Infection: Selective Increases in Monomer and Apparent Restriction of the Antibody Response to IgA1 Antibodies Mainly Directed atenvGlycoproteinsAIDS Research and Human Retroviruses, 1992