Human apolipoprotein B: partial amino acid sequence
Open Access
- 7 May 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 170 (1) , 105-108
- https://doi.org/10.1016/0014-5793(84)81378-2
Abstract
A successful approach has been developed for the sequencing of apolipoprotein B based upon the procedure of Cleveland et al. [(1977) J. Biol. Chem. 252, 1102–1106] involving limited proteolysis in the presence of sodium dodecyl sulfate. Staphylococcus aureus protease was employed to produce large peptides which were isolated in relatively pure form by preparative gel electrophoresis. Two peptides were partially sequenced using spinning‐cup microsequencing techniques. The sequences are: Peptide R2‐5, ‐Ala ‐ Leu ‐ Val ‐ Gly ‐ Ile ‐ Asn ‐ Gly ‐ Glu ‐ Ala ‐ Asn ‐ Leu ‐ Asp ‐ Phe ‐ Leu ‐ Asn ‐ Ile ‐ Pro ‐ Leu ‐ Arg‐ Ile ‐ Pro‐ Pro‐Met‐Arg‐(Arg)‐; and Peptide R3‐1, ‐Leu‐Val‐Ala‐Lys‐Pro‐Ser‐Val‐Ser‐Val‐Glu‐Phe‐Val‐Thr ‐Asn‐Met‐Gly‐Ile‐Ile‐Ile‐Pro‐Lys‐Phe‐Ala‐Arg‐. Several stretches of residues suitable for the construction of oligonucleotide probes have been identified.Keywords
This publication has 15 references indexed in Scilit:
- Monoclonal antibodies against human low density lipoproteinFEBS Letters, 1982
- Microsequence analysis of peptides and proteinsAnalytical Biochemistry, 1982
- Microsequence analysis of peptides and proteinsAnalytical Biochemistry, 1982
- Microsequence analysis of peptides and proteinsAnalytical Biochemistry, 1982
- A rapid and mild procedure for the isolation of DNA from mammalian cellsAnalytical Biochemistry, 1982
- Apolipoprotein B variant derived from rat intestine.Proceedings of the National Academy of Sciences, 1980
- STUDIES ON THE PRIMARY STRUCTURE OF APOLIPOPROTEIN BAnnals of the New York Academy of Sciences, 1980
- Heterogeneity of apolipoprotein B: isolation of a new species from human chylomicrons.Proceedings of the National Academy of Sciences, 1980
- The Plasma Lipoproteins: Structure and MetabolismAnnual Review of Biochemistry, 1978
- Lipoproteins: Structure and FunctionAnnual Review of Biochemistry, 1975