A Direct Spectrophotometric Microassay for Sulfated Glycosaminoglycans in Cartilage Cultures
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Connective Tissue Research
- Vol. 9 (4) , 247-248
- https://doi.org/10.3109/03008208209160269
Abstract
A rapid spectrophotometric procedure is described for the estimation of sulfated glycosaminoglycans in cartilage cultures. Papain digestion of tissue or culture medium provides glycosaminoglycans in solution for assay; an aliquot of the digest is mixed with the dye 1,9-dimethylmethylene blue. The assay is based on the metachromatic shift in absorption maximum which occurs when the dye is complexed with sulfated glycosaminoglycans. The reagent is stable, and the method is substantially free from interference, is sensitive to less than 1 microgram (4 micrograms/ml) of chondroitin sulfate, and provides a simple alternative to the traditional methods for glycosaminoglycan determinations.Keywords
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