Luteovirus Relationships Assessed by cDNA Clones from Barley Yellow Dwarf Viruses

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Abstract
The results of reciprocal dot blot hybridization tests using genomically mapped cDNAs derived from cultures of P-PAV, MAV-PS1, and NY-RPV, three isolates of barley yellow dwarf virus (BYDV), were consistent with their grouping by serology and other characteristics. Thus, cDNAs from the nonimmunogenic regions of the genomes of P-PAV and MAV-PS1 reacted homologously and also heterologously. MAV-PS1 cDNAs from the nonimmunogenic region also weakly detected a related BYDV type, namely NY-SGV. However, representative cDNAs derived from the immunogenic regions of the MAV-PS1 or P-PAV genomes reacted significantly only to the homologous viruses. None of the MAV-PS1 or P-PAV-derived cDNAs tested detected the NY-RPV isolate although they represented most of the viral genome. Also, no cDNAs derived from any part of the NY-RPV genome detected either MAV-PS1 or P-PAV. In tests of infected leaf samples, cDNAs representative of the MAV-PS1, P-PAV, or NY-RPV genomes did not detect any other luteoviruses tested.