Lipoprotein 28, an inner membrane protein of Escherichia coli encoded by nlpA, is not essential for growth
Open Access
- 31 July 1988
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 170 (8) , 3747-3749
- https://doi.org/10.1128/jb.170.8.3747-3749.1988
Abstract
Lipoprotein 28, an inner membrane protein of Escherichia coli encoded by nlpA, was found to be nonessential for cell growth. A deletion strain in which most of the coding region of the nlpA gene was replaced by a kanamycin resistance gene was able to grow under various conditions. No discernible differences between the wild type and the deletion strain were observed in terms of cell morphology and sensitivity to various chemicals, except for kanamycin.This publication has 12 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Oligonucleotide-Directed Site-Specific Mutagenesis Using Double-Stranded Plasmid DNAPublished by Elsevier ,1987
- Lipoprotein-28, a cytoplasmic membrane lipoprotein from Escherichia coli. Cloning, DNA sequence, and expression of its gene.Journal of Biological Chemistry, 1986
- A 37 × 103 molecular weight plasmid-encoded protein is required for replication and copy number control in the plasmid pSC101 and its temperature-sensitive derivative pHS1Journal of Molecular Biology, 1984
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Nucleotide sequence of the kanamycin resistance transposon Tn903Journal of Molecular Biology, 1981
- Characterization of new membrane lipoproteins and their precursors of Escherichia coli.Journal of Biological Chemistry, 1981