Metastatic potential of human colorectal carcinoma SW1222 cells transfected with cDNA encoding carcinoembryonic antigen
- 1 July 1994
- journal article
- Published by Springer Nature in Clinical & Experimental Metastasis
- Vol. 12 (4) , 324-328
- https://doi.org/10.1007/bf01753839
Abstract
In order to examine a role of carcinoembryonic antigen (CEA) in metastasis, cDNA encoding CEA was introduced into a clone of human colorectal carcinoma SW1222 cells. Western blot analysis revealed that all transfectants express CEA of 180 kDa while the parent clone does not. In the transfectants, the level of CEA expression in clone 3 was higher than that of clone 1. Clone 3 formed aggregates rapidly after suspended by trypsinization while clone 1 did not. In experimental metastasis assay where tumor cells were injected intrasplenically, clone 3 exhibited a higher liver-metastatic activity than clone 1. Fab fragment of anti-CEA antibody significantly inhibited both the cell aggregation and the liver metastases caused by clone 3. These findings suggested that CEA expressed on the cell surface may play an important role in hepatic metastasis from colorectal carcinoma, possibly through its cell adhesion activity.Keywords
This publication has 24 references indexed in Scilit:
- Cell adhesion activity of non-specific cross-reacting antigen (NCA) and carcinoembryonic antigen (CEA) expressed on CHO cell surface: Homophilic and heterophilic adhesionPublished by Elsevier ,2004
- The structure, metabolism and function of the carcinoembryonic antigen gene familyBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1990
- Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion moleculePublished by Elsevier ,1989
- Metastasis Inhibition of Different Tumor Types by Purified Laminin Fragments and a Heparin-Binding Fragment of FibronectinJNCI Journal of the National Cancer Institute, 1988
- The carcinoembryonic antigen (CEA) contains multiple immunoglobulin-like domainsBiochemical and Biophysical Research Communications, 1987
- Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequenceBiochemical and Biophysical Research Communications, 1986
- Malignant melanoma cell lines selected in vitro for increased homotypic adhesion properties have increased experimental metastatic potentialClinical & Experimental Metastasis, 1986
- A Synthetic Peptide from Fibronectin Inhibits Experimental Metastasis of Murine Melanoma CellsScience, 1986
- The hepatic clearance of circulating native and asialo carcinoembryonic antigen by the ratBiochemical and Biophysical Research Communications, 1975
- DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUESThe Journal of Experimental Medicine, 1965