Silencing of filamin A gene expression inhibits Ca2+‐sensing receptor signaling
- 24 February 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (7) , 1795-1800
- https://doi.org/10.1016/j.febslet.2006.02.035
Abstract
Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+‐sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.Keywords
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