Effects of erythropoietin injection on the anemic rats with different serum erythropoietin titer.

Abstract
The effects of administration of purified erythropoietin (EPO) were investigated in four types of anemic model rats made by the injection of phenylhydrazine, iron deficiency, X-ray irradiation and partial nephrectomy. Serum EPO titers of those anemic rats were determined by radioimmunoassay. Partially nephrectomized rats showed chronically uremic anemia and their serum EPO titer remained decreased. These rats were recovered from anemia by injecting EPO. On the other hand, serum EPO titer of other types of anemic rats increased with the progress of anemia and the serum EPO titer showed reverse correlation with hemoglobin concentration. In these rats, curative effect of injections of EPO on anemia was not observed. All those results show that injection of EPO has therapeutic effect on anemia in the animals with renal failure and low serum EPO titer. However, injections of EPO did not show any effect on anemia in the animal with regular renal functions because of their own homeostatic EPO productions under anemic condition.

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