The "inner layer" of the empty cell wall (the protoplast having flowed out through a cut end) gave no reaction with the cellulose reagents (iodine KI and 70% H2SO4), but the end at the cut gave a faint cellulose test. The author suggests that the cell wall is of 3 layers: outer, of pectic compounds; middle, of cellulose; and innermost, extremely thin, of "pectose substances or of cellulose heavily impregnated with pectose.".