Potent inhibition of hepatitis B virus production in vitro by modified pyrimidine nucleosides
Open Access
- 1 October 1990
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 34 (10) , 1986-1990
- https://doi.org/10.1128/aac.34.10.1986
Abstract
2',3'-Dideoxy-3'-fluorothymidine (FddThd), 2',3'-didehydro-2',3'- dideoxythymidine (ddeThd), and 3'-fluoro-5-methyl-deoxycytidine (FddMeCyt) are, in their triphosphate forms, selective inhibitors of human immunodeficiency virus type 1 reverse transcriptase. We report that 0.3 microM FddThd, FddMeCyt, or ddeThd as well as 3'-chloro-5-methyl-deoxycytidine (ClddMeCyt) or 3'-amino-5-methyl-deoxycytidine (AddMeCyt) almost completely blocked production of hepatitis B virus (HBV) particles by HBV DNA-transfected cell line 2.2.15 in vitro. Only at an at least 10-fold-higher concentration was a cytotoxic effect observed. These results indicate that FddThd, FddMeCyt, ClddMeCyt, AddMeCyt, and ddeThd are potent anti-HBV agents in vitro.This publication has 33 references indexed in Scilit:
- Selective extraction of polyoma DNA from infected mouse cell culturesPublished by Elsevier ,2004
- Inhibition of hepatitis B virus DNA polymerase by 3′‐fluorothymidine triphosphate and other modified nucleoside triphosphate analogsJournal of Medical Virology, 1990
- Inhibition of duck hepatitis B virus replication by purine 2′,3′-dideoxynucleosidesBiochemical and Biophysical Research Communications, 1988
- Phosphorylation, anti-HIV activity and cytotoxicity of 3′-fluorothymidineBiochemical and Biophysical Research Communications, 1988
- Inhibition of HIV-associated reverse transcriptase by sugar-modified derivatives of thymidine 5′-triphosphate in comparison to cellular DNA polymerases α and βBiochemical and Biophysical Research Communications, 1987
- Potentiation of the cytostatic effect of bleomycin on L5178y mouse lymphoma cells by pepleomycinEuropean Journal of Cancer and Clinical Oncology, 1983
- Suramin: A potent inhibitor of the reverse transcriptase of RNA tumor virusesCancer Letters, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Action of 1-β-d-Arabinofuranosylcytosine on mammalian tumor cells—2Published by Elsevier ,1972
- Recent developments in the measurement of nucleic acids in biological materials. A supplementary reviewThe Analyst, 1966