Gas-liquid partition chromatography: the separation of volatile aliphatic amines and of the homologues of pyridine

Publisher Website
Google Scholar
Abstract
A straight -line relationship is obtained when the logarithms of the retention volumes are plotted against the number of C atoms for each type of primary, secondary and tertiary amine. By means of this relationship the relative retention vol. of any amine may be predicted provided the retention vol. of any other member in the correct homologous series is known. The utilization of columns having polar and non-polar liquid phases to determine whether an unknown amine is primary, secondary or tertiary is descr. A plot of the relative retention volumes of primary, secondary and tertiary amines on paraffin against those obtained with "Lubrol MO" liquid phases shows the values for each type of amine to fall on straight lines of different slope. Of 27 amines containing 1-12 C atoms only 2 pairs (iso- and sec-butylamino; diiso- and disecbutylamines) cannot be separated on 4 ft. columns. The separation of the homologs of pyridine is described as a suitable method for the approx. standardization of pyridine bases for chromatographic use.