The Production of Typhoid Antibodies in Rabbits with the Use of Living Typhoid Organisms Adsorbed on Aluminium Cream

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Abstract
In the preparation of antiserums for bacterial organisms one difficulty lies in the preparation of an effective antigen. If the organism is killed by heat or by chemical means, the native protein is partially or completely denatured. The resultant antiserum is usually not a straight antiserum for the characteristic protein of the bacterial organism. We have found that live organisms can be quantitatively adsorbed on aluminium cream and that when so adsorbed can be injected intramuscularly into animals without producing any deleterious effects, even when these organisms are pathogenic for the animal injected. Procedure. Typhoid organisms isolated from an epidemic strain (Manteno) were grown on agar in Kolle flasks at 37 C. for 48 to 72 hours. The bacteria were washed from the agar with the smallest possible volume of sterile 0.85 per cent salt solution. The resultant suspension contained over 3 billion organisms per ml.

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