A novel importin α from rice, a component involved in the process of nuclear protein transport1
- 29 May 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 428 (3) , 259-262
- https://doi.org/10.1016/s0014-5793(98)00540-7
Abstract
In eukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin α. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin α. Although the encoded protein had only 23–27% amino acid identity to the importin αs from various organisms including plants, the fusion protein with glutathione S‐transferase showed a specific binding activity to the NLS of SV40 T‐antigen. These results suggest that the rice #61L protein is a novel importin α in plants.Keywords
This publication has 33 references indexed in Scilit:
- Importin α- from Arabidopsis thaliana Is a Nuclear Import Receptor That Recognizes Three Classes of Import SignalsPlant Physiology, 1997
- Cloning of a cDNA Encoding a Novel Importin-α Homologue, Qip1: Discrimination of Qip1 and Rch1 from hSrp1 by Their Ability to Interact with DNA Helicase Q1/RecQLBiochemical and Biophysical Research Communications, 1997
- A plant in vitro system for the nuclear import of proteinsThe Plant Journal, 1996
- Nuclear import in permeabilized protoplasts from higher plants has unique features.Plant Cell, 1996
- Nucleocytoplasmic TransportScience, 1996
- Taking from the cytoplasm and giving to the pore: soluble transport factors in nuclear protein importTrends in Cell Biology, 1995
- Isolation of a protein that is essential for the first step of nuclear protein importCell, 1994
- Phenotype of the fission yeast cell cycle regulatory mutant pim1‐46 is suppressed by a tobacco cDNA encoding a small, Ran‐like GTP‐binding proteinThe Plant Journal, 1994
- Nuclear targeting sequences — a consensus?Trends in Biochemical Sciences, 1991
- Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.The Journal of cell biology, 1990