The mechanistic plurality of cytochrome P-450 and its biological ramifications
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 14 (1-2) , 105-118
- https://doi.org/10.3109/00498258409151401
Abstract
The mechanistic plurality of the microsomal cytochrome P-450 enzyme system was illustrated by studies of the oxidative metabolism of benzo[a]pyrene, 3-hydroxybenzo[a]pyrene and arachidonic acid. Rat liver microsomal metabolism of benzo[a]pyrene or 3-hydroxy-benzo[a]pyrene, supported by cumene hydroperoxide, generated benzo[a]pyrene quinones via molecular O-dependent and -independent pathways. Arachidonic acid was metabolized by rat liver microsomal fractions to a variety of oxygenated products, including cis-trans diene conjugated monohydroxy acids, epoxyacids and .omega.- and .omega.-1-oxidation products. The chemistry of the different reaction products is discussed in terms of the possible mechanisms responsible for their formation and the role of the hemoprotein during catalysis. An integrated view for the reaction cycle of cytochrome P-450 was presented.This publication has 61 references indexed in Scilit:
- Epoxyeicosatrienoic acids stimulate glucagon and insulin release from isolated rat pancreatic isletsBiochemical and Biophysical Research Communications, 1983
- Novel epoxides formed during the liver cytochrome P-450 oxidation of arachidonic acidBiochemical and Biophysical Research Communications, 1982
- The oxidative metabolism of arachidonic acid by purified cytochromes P-450Biochemical and Biophysical Research Communications, 1981
- Peroxidatic oxidation of benzo[a]pyrene and prostaglandin biosynthesisBiochemistry, 1979
- The role of the superoxide anion in the xanthine oxidase-induced autoxidation of linoleic acidBiochemical and Biophysical Research Communications, 1978
- Aliphatic hydroxylation by highly purified liver microsomal cytochrome P-450. Evidence for a carbon radical intermediateBiochemical and Biophysical Research Communications, 1978
- Hydroperoxide catalyzed liver microsomal aromatic hydroxylation reactions involving cytochrome P-450Biochemical and Biophysical Research Communications, 1974
- A gel-electrophoretically homogeneous preparation of cytochrome P-450 from liver microsomes of phenobarbital-pretreated rabbitsBiochemical and Biophysical Research Communications, 1974
- A new spectral intermediate associated with cytochrome P-450 function in liver microsomesBiochemical and Biophysical Research Communications, 1971
- ADP-activated lipid peroxidation coupled to the TPNH oxidase system of microsomesBiochemical and Biophysical Research Communications, 1963