Structural studies of the Enterococcus faecalis SufU [Fe-S] cluster protein
Open Access
- 2 February 2009
- journal article
- Published by Springer Nature in BMC Biochemistry
- Vol. 10 (1) , 3
- https://doi.org/10.1186/1471-2091-10-3
Abstract
Iron-sulfur clusters are ubiquitous and evolutionarily ancient inorganic prosthetic groups, the biosynthesis of which depends on complex protein machineries. Three distinct assembly systems involved in the maturation of cellular Fe-S proteins have been determined, designated the NIF, ISC and SUF systems. Although well described in several organisms, these machineries are poorly understood in Gram-positive bacteria. Within the Firmicutes phylum, the Enterococcus spp. genus have recently assumed importance in clinical microbiology being considered as emerging pathogens for humans, wherein Enterococcus faecalis represents the major species associated with nosocomial infections. The aim of this study was to carry out a phylogenetic analysis in Enterococcus faecalis V583 and a structural and conformational characterisation of it SufU protein.Keywords
This publication has 43 references indexed in Scilit:
- Structural Refinement of Membrane Proteins by Restrained Molecular Dynamics and Solvent Accessibility DataBiophysical Journal, 2008
- In vivo iron–sulfur cluster formationProceedings of the National Academy of Sciences, 2008
- Maturation of Iron-Sulfur Proteins in Eukaryotes: Mechanisms, Connected Processes, and DiseasesAnnual Review of Biochemistry, 2008
- HscA and HscB Stimulate [2Fe-2S] Cluster Transfer from IscU to Apoferredoxin in an ATP-Dependent ReactionBiochemistry, 2006
- Mechanisms of iron–sulfur cluster assembly: the SUF machineryJBIC Journal of Biological Inorganic Chemistry, 2005
- Solution NMR Structure of the Iron–Sulfur Cluster Assembly Protein U (IscU) with Zinc Bound at the Active SiteJournal of Molecular Biology, 2004
- SWISS‐MODEL and the Swiss‐Pdb Viewer: An environment for comparative protein modelingElectrophoresis, 1997
- GROMACS: A message-passing parallel molecular dynamics implementationComputer Physics Communications, 1995
- Molecular dynamics with coupling to an external bathThe Journal of Chemical Physics, 1984
- Dictionary of protein secondary structure: Pattern recognition of hydrogen‐bonded and geometrical featuresBiopolymers, 1983