Enzymic determination of cholesterol in serum lipoproteins separated by electrophoresis.
Open Access
- 1 July 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (7) , 1116-1120
- https://doi.org/10.1093/clinchem/24.7.1116
Abstract
We describe a reliable, straightforward procedure for measuring the cholesterol content of serum lipoproteins separated by electrophoresis on cellulose acetate. The lipoprotein fractions are made visible by use of an enzymic reagent and then quantitated by densitometry. Standard electrophoresis equipment is used and the procedure requires just over 1 h for completion. All major fractions except chylomicrons are detected readily. Accuracy and precision agree favorably with results reported for generally accepted methods. Concentration and color development are linearly related to cholesterol concentrations up to 1.5 g/liter in a given lipoprotein fraction. We recommend this method for use in assessing high-density lipoprotein cholesterol and as an aid in lipoprotein phenotyping.This publication has 5 references indexed in Scilit:
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- HDL cholesterol and other lipids in coronary heart disease. The cooperative lipoprotein phenotyping study.Circulation, 1977
- High density lipoprotein as a protective factor against coronary heart diseaseThe American Journal of Medicine, 1977
- Serum Cholesterol, Lipoproteins, and the Risk of Coronary Heart DiseaseAnnals of Internal Medicine, 1971
- Fat Transport in Lipoproteins — An Integrated Approach to Mechanisms and DisordersNew England Journal of Medicine, 1967