DEMONSTRATION OF MEMBRANE-ASSOCIATED AND ORIENTED MICROFILAMENTS IN AMOEBA-PROTEUS BY MEANS OF A SCHIFF BASE GLUTARALDEHYDE FIXATIVE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 18  (1) , 95-106
Abstract
After fixation with a reaction product of glutaraldehyde and spermidine phosphate A. proteus cells show a network of cortical microfilaments and oriented bundles of thick and thin filaments. The cortical filament network appears to be membrane-attached and extends beneath the whole cytoplasmic membrane surface. In the uroid region and in retracting pseudopods the cortical layer is thicker than in advancing cell regions. Filament bundles are located predominantly in the ectoplasmic tube within the cortical network. They strictly parallel the cell surface contours.