Comparative studies of fatty acid synthases of corynebacteria.

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Abstract
Fatty acid synthases were isolated from 6 representative strains of corynebacteria and their properties were investigated. All synthases were multifunctional proteins (type I synthase). Fatty acid synthases of Corynebacterium glutamicum CNF 016 and Brevibacterium ammoniagenes CNF 096 produced saturated fatty acids (palmitic and stearic acids) and oleic acid. Synthases of C. diphtheriae CNF 017, C. xerosis CNF 010, C. equi CNF 002 and C. fascians CNF 006 synthesized exclusively saturated fatty acids. The Km values for acetyl-CoA of the enzymes from C. glutamicum CNF 016, B. ammoniagenes CNF 096, C. diphtheriae CNF 017, C. xerosis CNF 010, C. equi CNF 002 and C. fascians CNF 006 were 1.3, 11, 24, 80, 53 and 220 .mu.M, respectively. There were almost no differences among the 6 strains of corynebacteria in the Km values of other substrates. The properties of fatty acid synthase may be useful for the taxonomic characterization of corynebacteria.