Insertional inactivation of a gene which controls expression of vancomycin resistance on plasmid pHKK100
- 1 April 1992
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 92 (1) , 11-14
- https://doi.org/10.1016/0378-1097(92)90533-t
Abstract
Expression of inducible high level vancomycin resistance (Vmr) in enterococci appears to require other plasmid-encoded genes in addition to the previously described structural genes vanA and vanH . Tn 917 mutagenesis was used to identify such a region in the Vmr plasmid pHKK100. Insertional inactivation of a 693-bp open reading frame upstream from vanH resulted in complete loss of Vmr. This putative 26 642-Da protein has been designated VanR.Keywords
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