Characterization of a Developmentally Early Macrophage Progenitor Found in Normal Mouse Marrow

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Abstract
In tissue culture high proliferation potential colony forming cells [HPP-CFC] formed large colonies in which macrophages predominated. Granulocyte/macrophage colony forming cells (GM-CFC) formed smaller colonies of granulocytes and macrophages. HPP-CFC from normal adult mouse bone marrow were compared with GM-CFC from the same source. HPP-CFC differed from GM-CFC in the following ways: HPP-CFC formed larger colonies containing more than 5 .times. 104 cells. They required spleen conditioned medium as well as pregnant mouse uterine extract as stimulants. Only 9% of HPP-CFC were killed by the phase specific drug hydroxyurea, and 36-55% of GM-CFC were killed by the same treatment. The modal density of HPP-CFC was 1.070 g/cm3. That of GM-CFC was 1.080 g/cm3.