Thymine at —5 Is Crucial for cpc Promoter Activity of Synechocystis sp. Strain PCC 6714
Open Access
- 1 November 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (21) , 6477-6480
- https://doi.org/10.1128/jb.185.21.6477-6480.2003
Abstract
The levels of transcripts of the cpc operon were highly reduced in a PD-1 mutant of cyanobacterium Synechocystis sp. strain PCC 6714. This was due to a substitution of C for T that occurred at 5 bp upstream of the transcription initiation site of the cpc operon. Any substitution for T at the −5 position drastically reduced both in vivo and in vitro promoter activity in cyanobacterium Synechococcus sp. strain PCC 7942 but not the in vivo activity in Escherichia coli . This suggests that the requirement of −5T appears to be specific for a cyanobacterial RNA polymerase-promoter combination.Keywords
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