Intracellular Location of Beet Curly Top Virus Antigen as Revealed by Fluorescent Antibody Staining

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Abstract
The intracellular location of beet curly top virus (BCTV) antigen was studied by using the fluorescent antibody method on paraffin sections and enzyme-separated cells of sugar beet [Beta vulgaris] hypocotyl. Infected cells were examined from tissue collected daily, beginning 1 day after vein-clearing symptoms first were observed. The percentage of infected cells with detectable antigen restricted to the nucleus was high in tissue from plants collected shortly after vein-clearing appeared. The percentage of infected cells with antigen in both the nucleus and cytoplasm increased progressively after initial symptom development. BCTV initially is located in the nucleus but subsequently is distributed throughout the cytoplasm.