Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells
- 1 March 1977
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 3 (2) , 157-172
- https://doi.org/10.1007/bf01551812
Abstract
Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.Keywords
This publication has 20 references indexed in Scilit:
- The control of serum protein synthesis in hepatoma-fibroblast hybridsCell, 1975
- USE OF GLOBIN cDNA AS A HYBRIDIZATION PROBE FOR GLOBIN mRNAAnnals of the New York Academy of Sciences, 1974
- IN SITU LOCALIZATION OF GLOBIN MESSENGER RNA FORMATIONThe Journal of cell biology, 1974
- Human Serum Albumin Phenotype Activation in Mouse Hepatoma-Human Leukocyte Cell HybridsScience, 1974
- Haemoglobin synthesis in inducible, uninducible and hybrid friend cell clonesExperimental Cell Research, 1974
- A REVIEW OF ENZYME POLYMORPHISM, LINKAGE AND ELECTROPHORETIC CONDITIONS FOR MOUSE AND SOMATIC CELL HYBRIDS IN STARCH GELSJournal of Histochemistry & Cytochemistry, 1973
- Expression of liver phenotypes in cultured mouse hepatoma cells: Synthesis and secretion of serum albuminDevelopmental Biology, 1973
- On the mechanism of erythropoietin-induced differentiation: VI. Induced accumulation of iron by marrow cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964
- CLONAL GROWTH OF MAMMALIAN CELLS IN VITROThe Journal of Experimental Medicine, 1956