Laser‐induced desorption of proteins

1 August 1989

journal article
research article
Published by Wiley in Rapid Communications in Mass Spectrometry

Vol. 3 (8) , 259-263
https://doi.org/10.1002/rcm.1290030804

Abstract

Laser-induced desorption mass spectrometry has been applied to a number of proteins in the mass range 5000–150 000 u. The beam from an excimer-laser-pumped dye-laser at 266 nm has been focused to a spot of about 50 μm in diameter with irradiances in the 10⁷W/cm² region. A linear time-of-flight mass spectrometer has been used for mass spectrometric measurements, where positive and negative secondary ions of large proteins have been studied. The effect of different experimental parameters on the protein ion-signal intensities are discussed.

Keywords

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