Abstract
The air-drying technique and aceto-orcein stain were used on uncultured kidney cells from juvenile maskinonge, Esox masquinongy, to determine the chromosome number and karyotype. A somatic chromosome number of 2n = 50 was found in 88% of 200 well-spread colchicine metaphases. Karyotype analysis showed that all the chromosomes have their centromeres located in the terminal region. Chromosomal polymorphisms reported by others for kidney cells of fish were not observed in this study.