Transfection of a human gene that corrects the Lec1 glycosylation defect: evidence for transfer of the structural gene for N-acetylglucosaminyltransferase I.
- 1 December 1989
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 9 (12) , 5713-5717
- https://doi.org/10.1128/mcb.9.12.5713
Abstract
Chinese hamster ovary (CHO) glycosylation mutants provide an approach to cloning mammalian glycosyltransferases by transfection and gene rescue. In this paper, complementation of the lec1 CHO mutation by human DNA is described. Lec1 transfectants expressed human N-acetylglucosaminyltransferase I (GlcNAc-TI) activity and possessed common human DNA fragments. Cloning of GlcNAc-TI should therefore be possible. ImagesThis publication has 21 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- A CLONED HUMAN DNA RESTRICTION FRAGMENT DETERMINES EXPRESSION OF A GDP-L-FUCOSE - BETA-D-GALACTOSIDE 2-ALPHA-L-FUCOSYLTRANSFERASE IN TRANSFECTED CELLS - EVIDENCE FOR ISOLATION AND TRANSFER OF THE HUMAN-H BLOOD-GROUP LOCUS1989
- DNA-mediated transformation of N-acetylglucosaminyltransferase I activity into an enzyme deficient cell lineBiochemical and Biophysical Research Communications, 1989
- STABLE EXPRESSION OF BLOOD GROUP-H DETERMINANTS AND GDP-L-FUCOSE - BETA-D-GALACTOSIDE 2-ALPHA-L-FUCOSYLTRANSFERASE IN MOUSE CELLS AFTER TRANSFECTION WITH HUMAN DNA1989
- Chapter 3 Analysis of the Synthesis, Intracellular Sorting, and Function of Glycoproteins Using a Mammalian Cell Mutant with Reversible Glycosylation DefectsPublished by Elsevier ,1989
- The GDP-fucose:N-acetylglucosaminide 3-alpha-L-fucosyltransferases of LEC11 and LEC12 Chinese hamster ovary mutants exhibit novel specificities for glycolipid substrates.Journal of Biological Chemistry, 1987
- Specific changes in the oligosaccharide moieties of VSV grown in different lectin-resistant CHO cellsCell, 1978
- Selection and characterization of chinese hamster ovary cells resistant to the cytotoxicity of lectinsIn Vitro Cellular & Developmental Biology - Plant, 1976
- The chromic chloride method of coupling antigens to erythrocytes: Definition of some important parametersJournal of Immunological Methods, 1976
- Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine--glycoprotein N-acetylglucosaminyltransferase activity.Proceedings of the National Academy of Sciences, 1975