Domain interactions in human plasminogen studied by proton NMR
- 14 January 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 278 (1) , 17-22
- https://doi.org/10.1016/0014-5793(91)80073-c
Abstract
The NMR spectrum of miniplasminogen (V443-plasminogen) under conditions of acidic pH reveals a subset of particularly well-resolved resonances whose chemical shift values are closely similar to those of isolated kringle 5. The temperature dependence of the spectrum indicates that this set of resonances disappears in a single cooperative unfolding transition appropriate for kringle 5, whilst other broader resonances from the protease domain persist to higher temperature. These results provide evidence for significant structural and motional independence of the kringle and protease domains in spite of the short linker between these domains. The NMR spectrum of Glu1-plasminogen is closely similar to that of miniplasminogen under the same conditions. This suggests that the domain independence observed in miniplasminogen is maintained in the intact moleculeKeywords
This publication has 20 references indexed in Scilit:
- Solution structure of the kringle 4 domain from human plasminogen by 1H nuclear magnetic resonance spectroscopy and distance geometryJournal of Molecular Biology, 1990
- Analysis of the aromatic 1H‐NMR spectrum of the kringle 5 domain from human plasminogenEuropean Journal of Biochemistry, 1988
- Isolation, purification and 1H-NMR characterization of a kringle 5 domain fragment from human plasminogenBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1987
- Molecular cloning and characterization of a full‐length cDNA clone for human plasminogenFEBS Letters, 1987
- Characterization of the low-field proton magnetic resonance spectrum of plasminogen kringle 4 via selective overhauser experiments in 1H2OBiochemistry, 1986
- Domains in human plasminogenJournal of Molecular Biology, 1984
- Improved spectral resolution in COSY 1H NMR spectra of proteins via double quantum filteringBiochemical and Biophysical Research Communications, 1983
- Fossils in the making, vertebrate taphonomy and palaeoecology: A. K. Behrensmeyer and A. P. Hill (Editors). Prehistoric Archaeology and Ecology Series. The University of Chicago Press, Chicago, Ill., 1980, 338 pp. £4.20, U.S. $9.10 (paperback)Palaeogeography, Palaeoclimatology, Palaeoecology, 1981
- Liver Biopsy InterpretationJournal of Clinical Pathology, 1981
- Multiple gene duplication in the evolution of plasminogen. Five regions of sequence homology with the two internally homologous structures in prothrombinFEBS Letters, 1976