Evaluation of the thymidine kinase (tk) locus as an insertion site in the highly attenuated vaccinia MVA strain
- 1 March 1996
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 141 (3-4) , 663-669
- https://doi.org/10.1007/bf01718324
Abstract
The highly attenuated ‘modified vaccinia Ankara’ (MVA) strain is a potential live vaccine vector. Insertional inactivation of the tk-gene resulted in viruses difficult to purify. Co-integration of a functional fowlpox virus tk-gene allowed easy generation of recombinants, indicating that the genetically stable tk-gene region is a suitable insertion site, if tk-gene activity is substituted.Keywords
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