Effect of temperature on the content of pigments of stored cottonseed

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Abstract
Summary: Three pure‐bred varieties of cottonseed,G. hirsutum, which were planted and grown under similar environmental conditions were stored at 38°, 77°, and 85°F. After determining the initial contents of lipids, nitrogen, moisture, gossypol, and gossypurpurin each lot of seed was stored at the different temperatures and analyzed periodically with respect to changes in pigmentation.The content of gossypurpurin was found to increase during storage in all of the samples. Its increase was proportional to the temperature and length of storage. On the other hand, gossypol decreased during storage of all samples.The antimony trichloride test for gossypol was found to be applicable only to extracts prepared from fresh cottonseed. During storage of the seed another yellow‐colored pigment(s) developed which could be separated from gossypol by alkaline extraction of the original chloroform extract of the stored seed. The alkali extractable portion of the chloroform extract gave a red‐colored antimony trichloride reaction product characteristic of gossypol.It is postulated that at least a fraction of the non‐acidic pigment(s) in the crude chloroform extracts obtained from stored cottonseed is diaminogossypol.