Liquid-chromatographic determination of amoxapine and 8-hydroxyamoxapine in human serum.
Open Access
- 1 October 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 28 (10) , 2154-2157
- https://doi.org/10.1093/clinchem/28.10.2154
Abstract
We describe a liquid-chromatographic procedure for amoxapine and 8-hydroxyamoxapine, its active metabolite, in serum. We used a mu-Bondapak C18 reversed-phase column and a mobile phase of acetonitrile/water (74/26 by vol) plus 26 microL of n-butylamine per liter. The compounds were measured at 254 nm, with 8-methoxyloxapine as internal standard. Necessary pre-analysis purification consisted of adsorbing the drug from serum onto extraction columns, eluting with 1-butanol/hexane (1/5 by vol), re-extracting into aqueous acid, and from that re-extracting again into the elution-solvent mixture. We prefer this procedure for monitoring both therapeutic and toxic concentrations of amoxapine, because parent drug and metabolite are measured separately.This publication has 1 reference indexed in Scilit:
- GLC Analysis of Loxapine, Amoxapine, and Their Metabolites in Serum and UrineJournal of Pharmaceutical Sciences, 1979