Control of Protein Synthesis in Temperature Acclimation. I. Characterization of Polypeptide Elongation Factor 1 of Toadfish Liver

Abstract
Polypeptide elongation factor 1 (EF-1) of toadfish liver has been characterized by the binding of aminoacyl-tRNA to ribosomes in vitro. The properties of high and low molecular weight EF-1 after 70-fold purification are compared. Levels of liver EF-1 in rat and toadfish and Arrhenius plots for the assay in vitro are analyzed in relation to the rate of chain elongation and its temperature dependency in vivo