Analysis of Free Amino Acids in Microbially Colonized Sandstone by Precolumn Phenyl Isothiocyanate Derivatization and High-Performance Liquid Chromatography
- 1 March 1991
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 57 (3) , 879-881
- https://doi.org/10.1128/aem.57.3.879-881.1991
Abstract
A fast, sensitive method for extraction and analysis of soluble free amino acids from microbially colonized sandstone is described. After precolumn phenyl isothiocyanate (PITC) derivatization, the PITC-amino acids were identified and quantified by reversed-phase high-performance liquid chromatography. This kind of analysis could be used to elucidate the role and function of amino acids in the nutrition of epi- and endolithic microorganisms active in biological weathering processes.Keywords
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