Role of supernumerary subunits in mitochondrial cytochrome c oxidase
Open Access
- 4 December 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 258 (2) , 285-288
- https://doi.org/10.1016/0014-5793(89)81675-8
Abstract
Role of supernumerary subunits of bovine heart cytochrome c oxidase has been investigated by examining the influence on the enzymatic activity of their removal by Chromatographic procedures or controlled digestion by trypsin. Is has been shown that partial proteolytic cleavage of subunit IV results in depression of respiratory activity and of redox‐linked proton translocation. Selective removal by gel‐filtration of subunit VIb has no significant influence on the redox and protonmotive activity of the oxidase.Keywords
This publication has 14 references indexed in Scilit:
- A Direct Repeat Is a Hotspot for Large-Scale Deletion of Human Mitochondrial DNAScience, 1989
- Protonic sidedness of the binuclear iron‐copper centre in cytochrome oxidaseFEBS Letters, 1988
- Orientation of the cytoplasmically made subunits of beef heart cytochrome c oxidase determined by protease digestion and antibody binding experimentsBiochemistry, 1988
- Studies on the oligomeric state of isolated cytochrome oxidase using cross-linking reagentsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1987
- Purification of all thirteen polypeptides of bovine heart cytochrome c oxidase from one aliquot of enzyme Characterization of bovine fetal heart cytochrome c oxidaseFEBS Letters, 1987
- Characteristics of the redox‐linked proton ejection in beef‐heart cytochrome c oxidase reconstituted in liposomesEuropean Journal of Biochemistry, 1987
- Topology of beef heart cytochrome c oxidase from studies on reconstituted membranesBiochemistry, 1984
- Separation of mammalian cytochrome c oxidase into 13 polypeptides by a sodium dodecyl sulfate-gel electrophoretic procedureAnalytical Biochemistry, 1983
- Studies on the Transmembrane Orientation of Cytochrome c Oxidase in Phospholipid VesiclesEuropean Journal of Biochemistry, 1982
- Proton translocation reactions in the respiratory chainsBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1976