ANTIBODY-MEDIATED SUPPRESSION OF TUMOR-GROWTH .1. SUPPRESSION BY MURINE IGG1 ISOLATED FROM ALLOANTISERUM

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 118  (2) , 489-493
Abstract
The tumor suppressive activity of murine IgG1 [immunoglobulin G1] antibody was studied in vivo. IgG1 was isolated from hyperimmune alloantisera against murine lymphoma 6C3HED cells by absorption with heat-killed, formalinized Staphylococcus aureus Cowan strain I, followed by DEAE ion exchange chromatography and Sephadex G-200 gel filtration chromatography. The isolated IgG1, which had no detectable IgM, IgA, IgG2a, IgG2b or IgG3, could suppress the growth of the tumor. DEAE and Sephadex G-200 column profiles of the in vivo tumor suppressive activity showed good correlation with the profiles of total IgG1 and anti-tumor antibody assayed for IgG1 H chains and .kappa. L chains. The IgG1 tumor suppressive activity was not diminished after heating at 56.degree. C for 30 min.