Vitamin B12-intrinsic factor studies with isolated perfused rat liver

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Abstract
Vitamin B12-intrinsic factor relationships have been studied with the isolated perfused rat liver technique. Approximately 20 times more vitamin B12 is absorbed by the liver in the presence of intrinsic factor than without it. Rapid and significant transfer of vitamin B12 from liver to bile has been observed. The dose-response curve for this system is similar to that reported for the absorption of vitamin B12 through the intestinal tract of pernicious anemia patients. The order and time of addition of vitamin B12 and intrinsic factor to the perfusing blood is critical for absorption of vitamin B12 by the liver. If vitamin B12 is added first, binding substances in the blood rapidly make vitamin B12 unavailable to intrinsic factor added subsequently. If intrinsic factor is added first, gradual inactivation or removal of intrinsic factor by the liver leads to a much slower decrease in efficiency of absorption of subsequently added vitamin B12. These effects are similar to those reported on absorption of vitamin B12 through the human intestinal tract.

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