Isolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus
Open Access
- 1 July 1992
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 94 (1-2) , 181-186
- https://doi.org/10.1016/0378-1097(92)90605-n
Abstract
A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dower 1 × 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 μM added FeCl3. Hydrolysis of the compound yielded alanine, ethanolamine, citric acid and 2-ketoglutaric acid. The 1H-NMR spectrum exhibited the presence of a residue from each of these components in the intact molecule. The fast-atom bombardment mass spectrum of the methyl ester derivative indicated a prominent ion at m/z 477, probably corresponding to [M + 1] ion. Other strains of V. parahaemolyticus were also found to produce this compound when grown in an iron-limited medium.Keywords
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