Studies on the Growth-Inhibiting and Radioprotective Effect of Cystamine, Cysteamine, and AET on Mammalian Cells in Tissue Culture

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Abstract
Cystamine, cysteamine, and AET in concentrations above 0.9 x 10-4 [image], 2 x 10-4 [image], and 7 x 10-6 [image], respectively, inhibited growth in monolayer cultures of strain L mouse fibroblasts. When cells were treated for 60 minutes only, concentrations of 2 x 10-3 [image] did not inhibit subsequent growth in monolayers or in suspensions. The cells were irradiated with Earle''s salt solution with X-rays after preincubation with the test substances for 25 min. Cystamine and cysteamine in concentrations up to 2 x lO-3 [image] did not protect against the X-ray induced growth inhibition in suspension culture. With 2 x 10-3 [image] AET significant protection against 500 r of X-rays was obtained. Studies were made of the cellular uptake and the spontaneous oxidation of the substances. Results suggest a significant relationship between the toxic and metabolic effects of the protective substances and their radioprotective action.

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