Identification of psbI and psbL gene products in cyanobacterial photosystem II reaction center preparation
- 17 July 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 251 (1-2) , 155-160
- https://doi.org/10.1016/0014-5793(89)81446-2
Abstract
Photosystem II (PS II) reaction center complex of higher plants was revealed to contain a new low‐molecular‐mass component of 4.8 kDa encoded by a chloroplast gene, psbI [(1988) FEBS Lett. 241, 99–104], in addition to D1, D2 and two subunits of cytochrome b‐559. Here we isolated a PS II reaction center complex from the thermophilic cyanobacterium, Synechococcus vulcanus, by column chromatography using Triton X‐100 and urea, and attempted to identify the same gene product. SDS gel electrophoresis of the cyanobacterial reaction center complex resolved three protein bands of 8, 5.4 and 5 kDa in addition to D1, D2 and a partially remaining 47 kDa protein. N‐terminal sequencing revealed that the 5 kDa band consisted of two components: one, having an open N‐terminus, was homologous to the psbL gene product of higher plants, and the other, having a blocked N‐terminus, was homologous to the psbI gene product of higher plants. The 8 kDa and 5.4 kDa were the large and small subunits of cytochrome b‐559, respectively. Based on these results, we conclude that the subunit structure of cyanobacterial PS II reaction center complex is essentially the same as that of higher plants except that the cyanobacterial psbL product is more tightly associated with the reaction center.Keywords
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