Improved resolution in 1H‐detected 1H‐15N correlation experiments
Open Access
- 25 September 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 255 (2) , 369-371
- https://doi.org/10.1016/0014-5793(89)81124-x
Abstract
The determination of protein structure by NMR is restricted at molecular masses above 10 kDa by overlapping resonances. One way of overcoming this problem is to label the protein with 15N. The conventional way to record 15N spectra is to use heteronuclear multiple‐quantum coherence. We present here an alternative approach based on 15N single‐quantum coherence. This is shown to have substantial advantages over the multiple‐quantum method, including better F1 resolution.Keywords
This publication has 19 references indexed in Scilit:
- A powerful method of sequential proton resonance assignment in proteins using relayed 15N‐1H multiple quantum coherence spectroscopyFEBS Letters, 1989
- Concerted two-dimensional NMR approaches to hydrogen-1, carbon-13, and nitrogen-15 resonance assignments in proteinsBiochemistry, 1989
- Line narrowing of amide proton resonances in 2D NMR spectra of proteinsJournal of the American Chemical Society, 1989
- Long‐range 15N‐1H correlation as an aid to sequential proton resonance assignment of proteins Application to the DNA‐binding protein ner from phage MuFEBS Letters, 1988
- Improved resolution and sensitivity in 1H-detected heteronuclear multiple-bond correlation spectroscopyJournal of Magnetic Resonance (1969), 1988
- Assignment of nitrogen-15 NMR signals in bovine pancreatic trypsin inhibitorJournal of the American Chemical Society, 1987
- 1H Nuclear magnetic resonance studies of an integral membrane protein: Subunit c of the F1F0 ATP synthaseJournal of Molecular Biology, 1987
- Correlation of proton and nitrogen-15 chemical shifts by multiple quantum NMRJournal of Magnetic Resonance (1969), 1983
- Coherence transfer by isotropic mixing: Application to proton correlation spectroscopyJournal of Magnetic Resonance (1969), 1983
- A two-dimensional nuclear overhauser experiment with pure absorption phase in four quadrantsJournal of Magnetic Resonance (1969), 1982