Technique for Preparing Histologic Sections of Dogs' and Rabbits' Eyes in Paraffin

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Abstract
Introduction We have recently encountered increasing interest in eye sectioning procedures amongst those who experiment in animals with therapeutic agents, and this report is intended to describe the technique which we have evolved. The description is based chiefly on canine eyes and partly on those of rabbits. The former species is almost invariably employed in the course of subacute tests on most drugs and has also been the subject of two reports on drug-induced blindness.1,2 The rabbit is chiefly employed for the testing of agents intended to be instilled into the conjunctival sac. The subsequent ability of the technician to prepare good sections depends on the prior proper enucleation and fixation of the globe. These techniques in animals have recently been described for dogs,3,4 are identical for rabbits, and hence need not be repeated here; what follows is based on the cited descriptions. We fix, embed, and

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