Modification of ribosomal RNA by ribosome-inactivating proteins from plants
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (4) , 1359-13
- https://doi.org/10.1093/nar/16.4.1359
Abstract
Footprinting experiments from DNase I digests of a 139-base-pair segment of pBR-322 DNA in the presence of either netropsin or actinomycin D were carried out. Plots of oligonucleotide concentration as a function of drug concentration were analyzed to study the enhancement in cleavage rates at .apprx. 30 sites, accompanying drug binding at other sites. The pattern of enhancements is not consistent with drug-induced DNA structural changes, but agrees with a redistribution mechanism involving DNase I. Since the total number of enzyme molecules per fragment remains unchanged, drug binding at some sites increases the enzyme concentration at other sites, giving rise to increased cleavage. The consequences of the redistribution mechanism for analysis of footprinting experiments are indicated.This publication has 21 references indexed in Scilit:
- Demonstration of the asymmetric effect of CC-1065 on local DNA structure using a site-directed adduct in a 117-base-pair fragment from M13mp1.Proceedings of the National Academy of Sciences, 1987
- Quantitative Footprinting Analysis of the Netropsin-DNA InteractionJournal of Biomolecular Structure and Dynamics, 1987
- Actinomycin D facilitates transition of AT domains in molecules of sequence (AT)nAGCT(AT)n to a DNAse I detectable alternating structureNucleic Acids Research, 1987
- Energetics of cooperative protein-DNA interactions: comparison between quantitative deoxyribonuclease footprint titration and filter bindingBiochemistry, 1986
- Structural specificities of five commonly used DNA nucleasesJournal of Molecular Biology, 1984
- DNA structural variations in the E. coli tyrT promoterCell, 1984
- Binding of actinomycin D to DNA revealed by DNase I footprintingFEBS Letters, 1983
- Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.Proceedings of the National Academy of Sciences, 1983
- Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II).Proceedings of the National Academy of Sciences, 1982
- The Stereochemistry of Actinomycin Binding to DNA and Its Implications in Molecular BiologyProgress in Nucleic Acid Research and Molecular Biology, 1973