ESTABLISHMENT OF MOUSE NEURO-BLASTOMA CLONE-E7 IN SERUM-FREE MEDIUM

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 33  (2) , 155-+
Abstract
Clonal populations were derived from a wild mouse neuroblastoma cell population C 1300 in a serum-free medium containing commercially available serum growth-promoting proteins (GPP). From among these lines the clonal population E 7 was chosen for further work because it displayed maximum spontaneous morphological differentiation. The neuroblastoma clonal population differs morphologically from the original population; it was defined both cytogenetically and by means of growth parameters. The cells of the neuroblastoma clone E 7 are hypertetraploid with 2 chromosome number models, 88 and .apprx. 180-200. The majority of telocentric chromosomes in metaphases with a modal number of 88 chromosomes are identical with the chromosomes of mouse diploid cells. The cell generation time is 22 h. The cells of the clonal population E 7 are highly sensitive to the action of ethanolamine, which induces morphological differentiation, so that the processes of 30% of the cells in the population are over 40 .mu.m long. Electrophysiological studies showed that the cells of the neuroblastoma clonal population E 7 retain the character of excitable cells and they are thus suitable for studying some of the properties of nervous tissue cells.

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