Glycyl-tRNA Synthetase: An Oligomeric Protein Containing Dissimilar Subunits
- 1 December 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (4) , 1967-1974
- https://doi.org/10.1073/pnas.67.4.1967
Abstract
Glycyl-tRNA synthetase from Escherichia coli is a tetramer containing two small (33,000 daltons) and two large (80,000 daltons) subunits. The subunits can be separated and then reassembled to form active enzyme. Genetically altered glycyl-tRNA synthetases are modified in either the large or the small subunit. Wild type activity can be reconstituted in vitro by mixing extracts containing enzymes with modifications in different subunits.Keywords
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